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| Biochemicals | ROS assays were carried out as described previously (Shang-Guan et al., 2018). Briefly, leaf discs (0.2 cm2 ) were incubated overnight in a 96-well plate with water, and 200 mM luminol (Sigma-Aldrich, Saint Louis, MO, USA), 20 mg/mL horseradish peroxidase (Sigma-Aldrich, Saint Louis, MO, USA), or 100 nM flg22 (GenScript, Nanjing, China) were then added. | Get A Quote |
Tomato stress-associated proteins (SAPs) belong to A20/AN1 zinc finger protein family， some of which have been shown to play important roles in plant stress responses. However， little is known about the functions and underlying molecular mechanisms of SAPs in plant immune responses. In the present study， we reported the function of tomato SlSAP3 in immunity to Pseudomonas syringae pv. tomato (Pst) DC3000. Silencing of SlSAP3 attenuated while overexpression of SlSAP3 in transgenic tomato increased immunity to Pst DC3000， accompanied with reduced and increased Pst DC3000-induced expression of SA signalling and defence genes， respectively. Flg22-induced reactive oxygen species (ROS) burst and expression ... More
